#6572 FLUORINATED GRAPHENE REDUCES PLATELET ACTIVATION AND ADHESION ON HEMODIALYSIS CATHETER

Abstract Background and Aims Catheter thrombosis remains one of the major complications hemodialysis. Superhydrophobic surfaces are suggested to be capable creating an energy barrier that prevents cells proteins from contacting with catheter surface directly, making less thrombotic. Fluorinated graphene (FG) is a superhydrophobic material. It has been applied create self-cleaning surfaces. also shows good biocompatibility. Based on these features FG, we prepared FG coating hemodialysis attempting reduce clinical thrombotic events. Method suspension was made in solution 95 wt.% ethanol, 5 deionized water 3 1H,1H,2H,2H-Perfluorooctyltriethoxysilane (POTS, C14H15F17O3Si). Immediately following plasma activation, segments 1 cm²(Gamcath, GDHK1325) were spray coated both inside outside lumen. Samples air-dried for later use. We tested hemolysis rate, platelet activation adhesion performances, partial thromboplastin time (PTT) FG-coated samples. Test performed isotonic extract direct contact fresh rabbit blood respectively. Platelet according ASTM F2888-19 standard assessed by scanning electron microscope. PTT assay F2382-18 standard. Results hydrophobic angle 150° (Figure 1a). reduced 83.5% per 100 μm² area avoided 35.86% loss when compared uncoated 1b, 1c). rate than 2% test 1d). The sample 88.92±1.31s versus 83.03±9.49s yet no statistical significance (P>0.05) 1e). Conclusion great hemocompatibility. Though significant improvement observed assay, still significantly adherent activated catheter, which seems promising Yet, more tests needed performed, antithrombotic ability fluid state needs further confirmed study.